| Group | Club/Team Name | Team ID | Head Coach |
| D21 | Beach United FC - 01 Beach United FC - Pumas |
03022-001AG0U-1247 | Thuba Nkomazana |
| D22 | Cainhoy Athletic Soccer Club CASC 13 Hurricanes Grey |
03006-001AG0U-2336 | Gabriela Acosta |
| D23 | Charleston United SC - 01 Charleston United '13 Girls Blue |
03002-001RG0U-0090 | Jaime Duran |
| D24 | Charleston United SC - 01 Charleston United '13 Girls White |
03002-001RG0U-0098 | Jaime Duran |
| D25 | James Island Youth SC JIYSC 2013 Girls Red Pettler |
03001-001AG0U-1132 | Olivia Pettler |
| D26 | Mt. Pleasant SC - 01 Mt. Pleasant - G10U |
03007-001RG0U-0006 | n/a |
| D27 | Daniel Island SA DISA Dash Jr. Academy Pool |
03030-001AG0U-0012 | Jeffrey Collins |